Distinguishing between male and female Afghan Hound puppies can be done by observing the distance between their genitals and anus.
To determine the sex of a puppy, look for the presence of a penis between the male's legs. The male puppy's belly will typically appear cleaner and covered with fur. Female puppies, on the other hand, will have flatter genitals and may have a few spots on their belly. These spots are where the mammary glands will later develop.
Carefully examine the puppy's abdomen and observe the distance between the genitals and the anus. In male puppies, the genitals will be further away from the anus, while in female puppies, they will be closer. The male puppy's genitals are located on the belly, while the female's are closer to the tail. Additionally, the male puppy's belly tends to be cleaner than the female's, with no fur covering the area.